Automated NGS Library Preparation Market Size, Share & Trends Analysis Report By Product (Instruments, Kits & Reagents), By Type, By Workflow, By Application, By End-use, By Region, And Segment Forecasts, 2025 - 2030

Market Size & Trends

The global automated NGS library preparation market size was estimated at USD 704.7 million in 2024 and is projected to grow at a CAGR of 13.34% from 2025 to 2030. The demand for genomic research in clinical diagnostics, drug discovery, and personalized medicine drives the automated NGS library preparation industry. The growing need for high-throughput sequencing in cancer genomics and rare disease studies accelerates automation adoption. Computerized workflows improve efficiency, reduce human error, and enhance reproducibility in sequencing experiments. The increasing use of whole-genome and targeted sequencing further fuels the need for automated solutions. The expansion of genomic databases and bioinformatics advancements also contribute to the industry's growth.

The COVID-19 pandemic served as a strategic accelerator for the automated NGS library preparation industry, driving rapid adoption of automation to meet unprecedented demands for high-throughput genomic testing. Manual processes proved insufficient for large-scale sequencing needs, prompting laboratories to invest in scalable, automated solutions that enhanced efficiency, consistency, and turnaround time. Heightened global focus on genomic surveillance, coupled with increased public and private funding, reinforced the value of automation in sustaining operations during workforce constraints. As a result, automation transitioned from a technical enhancement to a business-critical capability across clinical, academic, and commercial sequencing environments.

Precision medicine is revolutionizing healthcare by tailoring treatments to an individual’s genetic profile. Next-Generation Sequencing (NGS) advances allow for rapid and cost-effective genetic analysis. This approach enhances disease diagnosis, prognosis, and therapy selection. Cancer treatment, rare diseases, and pharmacogenomics particularly benefit from precision medicine. The demand for high-throughput sequencing is rising to meet clinical and research needs. This shift drives innovation in sequencing platforms and bioinformatics tools.

Automation in NGS library preparation improves efficiency, reproducibility, and throughput. Robotic systems streamline complex processes such as DNA/RNA extraction and sample preparation. AI-driven solutions optimize workflows by minimizing human error and maximizing precision. Automated liquid handling ensures consistency in reagent distribution and sample mixing. These advancements reduce turnaround time and costs for genomic research. As a result, high-throughput sequencing becomes more accessible and scalable.

Automation as a Catalyst in NGS Workflow Transformation

The integration of automation in next-generation sequencing (NGS) library preparation is revolutionizing public health and clinical diagnostics. As highlighted in the source, automated systems reduce human error, improve consistency, and enable higher throughput-key advantages in time-sensitive and resource-constrained settings. The COVID-19 pandemic underscored the importance of scalable sequencing infrastructure, accelerating the demand for automated workflows.

Despite challenges like initial costs and training requirements, laboratories increasingly recognize automation as a long-term investment. Automated liquid handlers and modular platforms streamline complex steps such as magnetic bead purification and enzymatic fragmentation. This transformation is driving sustainable adoption of advanced molecular diagnostics, positioning automated NGS library preparation as a cornerstone in the evolving genomics landscape. To capitalize on this trend, several key players have developed fully automated library preparation platforms as shown in the below figure.

Note: The above figure shows various fully automated library preparation platforms offered by key players, ranging from low-throughput systems (<96 samples) to mid- and high-throughput platforms (up to 384 samples).

As sequencing applications expand beyond research into clinical and epidemiological use, the role of automated library preparation becomes even more critical. The ability to process large sample volumes with minimal variability ensures reproducibility and data quality-essential for diagnostics, surveillance, and personalized medicine. Automation not only enhances efficiency but also supports the scalability required for population-level genomics, reinforcing its growing value across academic, commercial, and public health laboratories.

Advancements in Automation and Robotics

The shift toward automation in NGS library preparation is fundamentally altering how laboratories manage scale, efficiency, and cost. Automated platforms now handle complex, multi-step protocols-from nucleic acid extraction to adapter ligation-with minimal manual intervention. This reduces turnaround times and operational burden, allowing labs to redeploy skilled personnel to more analytical or interpretative roles. As demand grows for high-throughput sequencing in clinical and translational research, automation becomes a strategic imperative rather than a technical upgrade.

Furthermore, the integration of automation ensures standardization across runs, projects, and even across global sites-critical for regulatory compliance and large-scale collaborations. Automated systems are increasingly modular and adaptable, supporting diverse sample types and sequencing protocols. This flexibility allows institutions to future-proof their investments, align with evolving research priorities, and meet the rising expectations of data quality and reproducibility in modern genomics.

Market Concentration & Characteristics

Innovation in the automated NGS library preparation industry is driven by advancements in robotics, microfluidics, and AI-powered automation. Companies are focusing on enhancing workflow efficiency, reducing sample preparation time, and improving sequencing accuracy. Emerging innovations include integrated platforms that minimize human intervention and optimize reagent usage. In addition, improvements in software solutions are enabling better data analysis and interpretation.

The industry has witnessed significant M&A activity as larger sequencing companies acquire automation specialists to enhance their library preparation capabilities. Notable deals include acquisitions aimed at integrating automated liquid-handling technologies into NGS workflows. Strategic partnerships between biotech firms and sequencing providers are also accelerating, allowing for seamless automation solutions. These activities help companies expand their market presence and streamline product offerings.

Regulatory frameworks significantly influence product development and commercialization in the automated NGS library preparation industry. Organizations like the FDA and EMA enforce stringent guidelines to ensure accuracy, reproducibility, and compliance in clinical applications. Data security and privacy regulations also impact automated solutions, particularly in cloud-based and decentralized sequencing applications. Compliance challenges may affect the time-to-market for new products.

The automated NGS library preparation industry is seeing continuous product expansion, with companies launching automation platforms catering to high-throughput and low-input sequencing applications. New product lines are focusing on compatibility with various sequencing platforms, offering flexibility for researchers and clinicians. In addition, developments in pre-filled reagent kits and modular systems are simplifying workflows and making automation more accessible to smaller labs and research centers.

Leading companies in the automated NGS library preparation industry is expanding their presence across key regions to strengthen their global footprint. Companies such as Illumina and Thermo Fisher Scientific are investing in manufacturing and research facilities in Asia-Pacific to cater to the growing demand for sequencing automation. European expansion is driven by partnerships with clinical research organizations and government-funded genomics initiatives. In North America, companies are scaling operations through collaborations with biotech firms and research institutions, while emerging markets in Latin America and the Middle East are seeing increased distributor agreements and local partnerships to improve market accessibility.

Product Insights

The kits & reagents segment accounted for the largest market revenue share in 2024, driven by increasing demand for high-throughput sequencing and standardized workflows. Automation in library preparation has enhanced efficiency and reproducibility, leading to a higher adoption rate among research laboratories and clinical settings. The rising prevalence of genetic disorders and cancer has fueled the need for reliable and scalable sequencing solutions. Continuous advancements in reagent formulations have improved the accuracy and yield of sequencing libraries. The growing number of research projects requiring next-generation sequencing (NGS) has further contributed to the demand for ready-to-use kits.

The instruments segment is projected to grow at the fastest CAGR over the forecast period, fueled by the rising adoption of automation in genomics research and clinical diagnostics. Technological advancements have led to the development of compact and user-friendly instruments that enhance workflow efficiency. The increasing complexity of genomic data analysis has necessitated robust and high-performance library preparation systems. A surge in investments from pharmaceutical and biotechnology companies has accelerated innovation in automated platforms. The demand for faster turnaround times in sequencing workflows has further supported the adoption of advanced instruments.

Type Insights

Based on type, the targeted genome sequencing segment led the market with the largest revenue share of 45.76% in 2024, which can be attributed to its cost-effectiveness and high accuracy in analyzing specific genomic regions. This approach is widely used in oncology, rare disease research, and hereditary disease screening, where precise mutation detection is essential. The reduced sequencing cost and data processing requirements have made targeted sequencing an attractive choice for researchers. Advances in hybridization-based capture and amplicon sequencing have further improved the efficiency of targeted sequencing workflows. The increasing application of personalized medicine has strengthened the demand for focused and reliable sequencing methods.

The whole genome sequencing (WGS) segment is poised for the largest CAGR of 14.58% during the forecast period, fueled by its unmatched ability to deliver comprehensive genomic insights. As a powerful tool for identifying both coding and non-coding mutations, WGS is increasingly leveraged in the discovery of novel disease markers and complex genetic traits. The continued decline in sequencing costs, coupled with enhanced library preparation workflows, has significantly improved turnaround times and data quality-making WGS more commercially viable for both clinical diagnostics and large-scale research initiatives. With the growing emphasis on precision medicine and data-driven healthcare, WGS is rapidly emerging as a strategic investment area across biotech, pharmaceutical, and healthcare institutions.

Workflow Insights

Based on workflow, the nucleic acid extraction segment led the market with the largest revenue share of 34.30% in 2024, which can be attributed to its essential role in ensuring high-quality input material for downstream NGS applications. As the first and most critical step in the library preparation workflow, efficient extraction directly influences data accuracy and sequencing success. The adoption of automated magnetic bead-based technologies has enhanced throughput, minimized contamination risks, and improved consistency across samples. With rising demand for high-throughput sequencing in clinical and research settings, automated extraction solutions have become a priority investment, further solidifying this segment’s market growth.

The fragmentation segment is projected to grow at the fastest CAGR of 15.50% over the forecast period, driven by advancements in enzymatic fragmentation technologies and growing demand for high-throughput, automation-compatible workflows. Fragmentation is a pivotal step in library preparation, and improvements in precision and uniformity have enhanced sequencing efficiency and data quality. The shift from mechanical to enzymatic methods has reduced process time and instrument dependency, making it ideal for automated platforms. As NGS adoption expands across clinical and translational research, the need for scalable and reliable fragmentation solutions is expected to accelerate, positioning this segment as a key driver of market growth.

Application Insights

Based on application, the drug & biomarker discovery segment led the market with the largest revenue share of 38.58% in 2024, which can be attributed to the rising demand for targeted therapies and personalized treatment approaches. Automated NGS library preparation enables high-throughput screening of genetic variations associated with drug response and disease progression. Pharmaceutical companies are leveraging sequencing technologies to accelerate drug development and optimize clinical trial outcomes. The integration of genomic biomarkers in therapeutic decision-making has increased the need for reliable and scalable sequencing workflows. Advances in bioinformatics tools have further facilitated the identification of novel drug targets, strengthening market growth.

The disease diagnostics segment is projected to grow at the fastest CAGR of 14.39% over the forecast period, due to the increasing prevalence of genetic disorders, infectious diseases, and cancer. Automated NGS library preparation enhances the accuracy and efficiency of diagnostic sequencing, supporting early disease detection and personalized treatment strategies. The demand for rapid and cost-effective genomic testing has led to the widespread adoption of automated platforms in clinical laboratories. Improvements in sequencing technologies have enabled better identification of rare mutations and pathogenic variants. The growing shift toward precision medicine has further accelerated the use of NGS in disease diagnostics.

End-use Insights

Based on end use, the pharmaceutical and biotechnology companies segment led the market with the largest revenue share of 43.22% in 2024, fueled by the increasing use of genomic data in drug development and precision medicine. Automated NGS library preparation plays a crucial role in accelerating biomarker discovery and companion diagnostics. The rising demand for targeted therapies has led to a greater focus on high-throughput sequencing solutions. Advancements in automation have improved workflow efficiency, enabling faster and more cost-effective genomic analysis. Strategic collaborations between biotech firms and sequencing technology providers have further contributed to market expansion.

The academic and research institutions segment is projected to grow at the fastest CAGR over the forecast period, driven by extensive genomic research initiatives and collaborations. Universities and research centers are increasingly adopting automated NGS library preparation systems to enhance sequencing efficiency and data reproducibility. The growing focus on functional genomics, evolutionary studies, and disease mechanisms has increased the demand for high-throughput sequencing. Continuous technological advancements have enabled researchers to analyze complex genomic data with improved accuracy and efficiency. The availability of funding for large-scale genomics projects has further supported the widespread adoption of NGS technologies.

Regional Insights

North America dominates the automated NGS library preparation market with the largest revenue share of 39.12% in 2024, due to the strong adoption of advanced sequencing technologies in research and clinical applications. The presence of major industry players drives innovation, improving workflow efficiency and accuracy. Growing investments in genomics for drug discovery and personalized medicine fuel market expansion. The high prevalence of genetic disorders increases demand for automated solutions that streamline sample processing. The region's well-established healthcare infrastructure supports continuous advancements in sequencing automation.

U.S. Automated NGS Library Preparation Market Trends

The automated NGS library preparation market in the U.S. leads due to extensive research initiatives and the adoption of high-throughput sequencing in the biotechnology and pharmaceutical sectors. Expanding applications in oncology, reproductive health, and infectious disease diagnostics enhance market growth. The rising focus on reducing sequencing turnaround time accelerates demand for automated workflows. The presence of multiple sequencing service providers strengthens accessibility and adoption rates. Advancements in AI-driven automation improve reproducibility and minimize errors in library preparation.

Europe Automated NGS Library Preparation Market Trends

The automated NGS library preparation market in Europe experiences steady growth in due to rising genomic research and biopharmaceutical developments. Increased focus on precision medicine boosts demand for efficient sequencing workflows. Growing collaborations between academic institutions and industry players enhance market opportunities. Higher demand for cost-effective, high-throughput solutions drives automation adoption. Expanding clinical applications in rare disease diagnostics further accelerates market expansion.

The UK automated NGS library preparation market sees rising adoption of automated NGS library preparation due to increasing focus on genomic sequencing in healthcare and research. The strong presence of biotechnology firms fosters continuous innovation in sequencing automation. Expanding applications in hereditary disease screening and oncology strong market demand. Increasing use of sequencing in agriculture and microbiome research further propels growth. Continuous technological advancements improve data accuracy and workflow efficiency.

The automated NGS library preparation market in benefits from strong biomedical research initiatives and industrial R&D investments. The high demand for precision diagnostics in healthcare drives automation in sequencing workflows. The presence of major life sciences companies accelerates technological advancements. Expanding applications in pharmacogenomics and infectious disease surveillance enhance market potential. The growing need for high-throughput solutions supports continuous improvements in sequencing efficiency.

Asia Pacific Automated NGS Library Preparation Market Trends

The automated NGS library preparation market in Asia Pacific exhibits rapid growth due to increasing genomics research and healthcare investments. Rising cancer incidence fuels demand for advanced sequencing technologies. The expanding biopharmaceutical industry accelerates the adoption of automation for high-throughput analysis. The growing interest in personalized medicine strengthens market expansion. Improvements in sequencing infrastructure enhance the efficiency and scalability of library preparation workflows.

The China automated NGS library preparation market sees significant growth driven by an increasing focus on precision medicine and genetic research. The expanding biotech sector fuels the demand for automated sequencing workflows to enhance efficiency. The rising prevalence of chronic diseases accelerates the adoption of NGS technologies for diagnostics. Higher sequencing capacity in research institutes and commercial labs strengthens automation trends. Continuous innovation in bioinformatics and sequencing platforms enhances data quality and process reliability.

The automated NGS library preparation market in Japan benefits from advancements in genomics for personalized healthcare and drug development. Increasing applications in rare disease diagnostics boost demand for high-throughput sequencing solutions. The strong presence of technology-driven companies drives continuous improvements in automation. Rising interest in microbiome research and infectious disease surveillance supports market expansion. Focus on minimizing sequencing errors enhances the adoption of AI-based automation.

MEA Automated NGS Library Preparation Market Trends

The automated NGS library preparation market in the Middle East and Africa are projected to grow the adoption of automated NGS library preparation due to the increasing focus on genomics in healthcare and agriculture. Expanding research collaborations strengthen sequencing capabilities and automation trends. The rising burden of genetic disorders drives demand for efficient library preparation solutions. A growing interest in disease surveillance and precision medicine enhances market growth. Technological advancements improve sequencing accessibility and affordability in the region.

The Kuwait automated NGS library preparation market is anticipated to grow at a steady CAGR during the forecast period, due to rising interest in genomic medicine and personalized healthcare. Increasing demand for efficient sequencing solutions supports automation in library preparation. Expanding applications in cancer research and reproductive health boost market potential. Higher investments in biomedical research strengthen sequencing infrastructure. Improvements in sequencing accuracy and turnaround time drive automation adoption. 

Competitive Scenario Insights

The automated NGS library preparation industry is becoming increasingly competitive, marked by rapid innovation and strategic positioning across leading and emerging players. Illumina, Inc. and Thermo Fisher Scientific Inc. continue to lead the market with their robust sequencing platforms and automation-compatible library preparation systems, offering end-to-end solutions that support high-throughput and clinical-grade applications. Their dominance is reinforced through strategic acquisitions, platform integration, and global distribution networks, making them key influencers in setting industry benchmarks.

Agilent Technologies, QIAGEN N.V., and F. Hoffmann-La Roche Ltd maintain strong market positions through differentiated reagent portfolios, advanced sample prep technologies, and a focus on diagnostics-driven genomics workflows. These companies are enhancing automation capabilities to meet rising demands for efficiency, scalability, and consistency, especially in clinical and translational research settings.

Meanwhile, companies like Bio-Rad Laboratories, Inc., Revvity, and Hamilton Company are expanding their footprint by offering modular automation systems, liquid handling platforms, and workflow optimization tools. Innovators such as Oxford Nanopore Technologies and Pacific Biosciences of California, Inc. are gaining traction with next-generation long-read sequencing technologies, which require customized library preparation workflows. This presents new opportunities in precision medicine and population-scale genomics. This competitive mix is driving innovation and reshaping how laboratories adopt and scale automated NGS solutions.

Key Automated NGS Library Preparation Company Insights

Key players operating in the automated NGS library preparation industry are undertaking various initiatives to strengthen their market presence and increase the reach of their products and services. Strategies such as expansion activities and partnerships are key in propelling market growth.

Recent Developments

• In February 2025, seqWell announced the early access availability of its MosaiX Library Prep Kit for human population genomics applications such as WGS and whole exome sequencing. The kit featured seqWell’s TnX transposase, enabling improved activity and reduced insertion-site bias. It offered a 90-minute end-to-end workflow, producing PCR-ready libraries in just 45 minutes.

• In June 2024, SPT Labtech and Watchmaker Genomics partnered to simplify and streamline NGS library preparation by developing automated mRNA and DNA sequencing protocols.

• In September 2023, PacBio announced collaborations with automation providers Integra, Hamilton, Revvity, and Tecan-as part of the PacBio Compatible initiative. This initiative aimed to ensure the compatibility of products optimized for use in PacBio workflows, providing customers with automated protocols for library preparation workflows for whole genome sequencing. 

Automated NGS Library Preparation Market Report Scope

Global Automated NGS Library Preparation Market Report Segmentation

This report forecasts revenue growth at global, regional, and country levels and analyzes the latest industry trends in each of the sub-segments from 2018 to 2030. For this study, Grand View Research has segmented the global automated NGS library preparation market report based on product, type, application, workflow, end-use, and region:

• Product Outlook (Revenue, USD Billion, 2018 - 2030)

  • Instruments

  • Kits & Reagents

    • DNA Library Preparation Kits

    • RNA Library Preparation Kits

    • Exome/Targeted Capture Library Preparation Kits

    • Others           

• Type Outlook (Revenue, USD Billion, 2018 - 2030)

  • Targeted Genome Sequencing

  • Whole Genome Sequencing

  • Whole Exome Sequencing

  • Other Sequencing Types

• Workflow Outlook (Revenue, USD Billion, 2018 - 2030)

  • Nucleic Acid Extraction

  • Fragmentation

  • Adapter Ligation

  • Purification & Quantification

• Application Outlook (Revenue, USD Billion, 2018 - 2030)

  • Drug & Biomarker Discovery

  • Disease Diagnostics

    • Cancer Diagnostics

    • Reproductive Health Diagnostics

    • Infectious Disease Diagnostics

    • Other Disease Diagnostic Applications

  • Others

• End-use Outlook (Revenue, USD Billion, 2018 - 2030)

  • Hospitals and Clinics

  • Academic and Research Institutions

  • Pharmaceutical and Biotechnology Companies

  • Others

• Regional Outlook (Revenue, USD Billion, 2018 - 2030) 

  • North America

    • U.S.

    • Canada

    • Mexico

  • Europe

    • Germany

    • UK

    • France

    • Italy

    • Spain

    • Sweden

    • Denmark

    • Norway

  • Asia Pacific

    • China

    • Japan

    • India

    • South Korea

    • Australia

    • Thailand

  • Latin America

    • Brazil

    • Argentina

  • Middle East and Africa (MEA)

    • South Africa

    • UAE

    • Saudi Arabia

    • Kuwait